This thesis examines physiological markers of separation distress within Panksepp's Affective Neuroscience, focusing on the distinction between the PANIC and GRIEF systems with secondary discussion of the SEEKING system. Building on the CHOice and MOtivation (CHOMO) project, the study explores how these primary affective systems manifest in autonomic arousal, as indexed by Skin Conductance Responses (SCRs), during the perception of attachment-related emotional stimuli. Participants viewed images from the Attachment- Related Picture Set (ARPS) representing PANIC, GRIEF, and neutral conditions. SCR data were preprocessed and analyzed for phasic components to capture transient aspects of emotional arousal. It was hypothesized that PANIC-related stimuli, reflecting acute separation distress and hypervigilance, would elicit stronger phasic responses, whereas GRIEF-related stimuli, reflecting resignation and loss processing, would be associated with more subdued responses due to withdrawal and reduced autonomic activation. Individual differences in SCR response profiles were also correlated with emotional-system-related behavior through the ANPS-PGL questionnaire. The analysis revealed higher amplitudes for PANIC compared to GRIEF and neutral images, and lower conductance for the GRIEF condition than the neutral condition, aligning with theoretical predictions. Blunted SCR responses were found to be linked to differences in the activity of approach-and-affiliation related systems as measured by the ANPS-PGL, rather than the PANIC and GRIEF systems themselves. These findings support the differentiation of PANIC and GRIEF at the physiological level and provide empirical grounding for affective neuroscience models linking personality, emotion, and autonomic regulation.